In this potentially deadly region, the virus causing COVID-19 can only be studied in the following levels of biosecurity. The scientific knowledge that the infectious virus must be used is a complete biological risk of full body with controlled respirators and to work within laboratories with different levels of content of specialized ventilation systems. However, it is necessary to protect laboratory workers, retarding safety safeguards for the provision of medicines and vacancies for COVID-19, that many of the scientific requirements associated with the required biosecurity installations.
To help remediar eso, the investigators of the Faculty of Medicine of the University of Washington in St. Louis has developed a hybrid virus that permits the most sensitive to participate in the fight against the pandemic. The investigators genetically modified the virus caused by the Al intercambiar uno de sus genes provo del SARS-CoV-2, the virus that caused COVID-19. The resulting hybrid virus infects the cells and is detected by anticancer in the other that the SARS-CoV-2 can be treated under normal laboratory conditions.
El estudio is available in a range cell phone and microbio .
“He never had any requests for scientific material in this time,” said Sean Whelan, PhD, Professor Marvin A. Brennecke, Distinguished Professor, and head of the Department of Molecular Microbiology. “We distributed the virus to investigators in Argentina, Brazil, Mexico, Canada and, in addition, in all United States. We have the same requests from the United Kingdom and Germany. Incorporated of publicar, the escaping people who are working in this complex to solicit the material “
To create a model of SARS-CoV-2 that will be more important to manage, Whelan and its students, including principal co-author Michael S. Diamond, MD, PhD, professor of medicine Herbert S. Gasser and co-authors, Brett Case, PhD, a postdoctoral researcher at Diamond Laboratory, and Paul W. Rothlauf, a graduate student at Whelan Laboratory, comedian with the vesicular VSV estomatitis virus. This virus is an easy-to-control genetically manipulated bacterium that is easy to manipulate. Mainly the virus bovino ganado, equino y porcino, el VSV occasionally infected las personas, which caused it to enfermedad increased similarly to the gripe, which lasted from tres to cinco días.
Virus tienen proteins in addition to the areas used to engancharse and infect cellulases. Investigators will eliminate the gene of surface protein of VSV and reemplate it from the SARS-CoV-2, cognized as espiga. The interruptor created a new virus, which was directed to the cells as SARS-CoV-2, but cares for other necessary genes to cause serious illness. Hybrid virus Denominaron el VSV-SARS-CoV-2.
Using the COVID-19 survivors and purified antibodies, the investigators demonstrated that the hybrid virus was detected by the human antiperspirants who have a SARS-CoV-2 virus that actually causes a patient with COVID-19. Anticuerpos o sueros, which impidieron el virusel hybrid virus infecting cells tambourine blocked the real SARS-CoV-2 virus; the anticuses or serums that do not detect the hybrid virus tampoco lograron disintegrate the SARS-CoV-2 real. Admittedly, a secreted molecule can be effectively effective in causing the virus and possibly infecting the cells.
“Human rights will be clearly developed against other proteases of SARS-CoV-2, but are antitrust against those that are more significant for protection,” said Whelan. “Mention of virus such as protein, pareceel human immunization system as SARS-CoV-2, for all effects”
The hybrid virus can be used to help patients evaluate a variety of treatments and preventions based on anti-dumping measures for COVID-19. The virus can be used to evaluate and try to experimentally produce neutralizing anticuerposes, to measure if survivor of COVID-19 can be used to neutralize neutralizing anticuerposal plasma and patients with COVID-19, or to identify anticuerposes with potentially converting antiviral drugs.
“One of the problems in evaluating neutralizing anticodes is that many of these tests require a BSL-3 installation and the care of clinical laboratories and companies in the absence of BSL-3 installations,” says Diamond, who is also a professor of molecular demicrobiology. , and pathology and immunology. we are infected with SARS-CoV-2 very good. “
As the hybrid virus is associated with SARS-CoV-2 for the system, it may not cause serious injury, it is a possible candidate for the vacancy, the Diamond aggregate. Yes, Whelan and their students are realizing animal studies to evaluate the possibility.